| Name: | Mouse Monoclonal Antibody to Microtubule Associated Protein 2, MAP2A/B/C/D |
| Immunogen: | Full length recombinant human MAP2D protein Prot-r-MAP2D expressed in and purified from E. coli. |
| HGNC Name: | MAP2 |
| UniProt: | P11137 |
| Molecular Weight: | MAP2A/B ~280 kDa, MAP2C/D ~70kDa by SDS-PAGE |
| Host: | Mouse |
| Isotype: | IgG1 |
| Species Cross-Reactivity: | Human, Rat, Mouse |
| RRID: | AB_2572215 |
| Format: | Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3 |
| Applications: | WB, IF/ICC, IHC |
| Recommended Dilutions: | WB: 1:5,000-1:10,000. IF/ICC and IHC: 1:5,000-10,000. |
| Storage: | Stable at 4°C for one year, for longer term store at -20°C |
Immunofluorescent analysis of adult rat hippocampus section stained with mouse mAb to MAP2A/B/C/D, MCA-2C4, dilution 1:5,000 in green, and chicken pAb to FOX2, CPCA-FOX2, dilution 1:2,000, in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. MCA-2C4 antibody labels all MAP2 protein isotypes expressed in neuronal perikarya and dendrites. The FOX2 antibody stains the nuclei of most neuronal cells.
Western blot analysis of tissue and cell lysates using mouse mAb to MAP2C/D, MCA-2C4, dilution 1:5,000 in green, and chicken pAb to vimentin, CPCA-Vim, dilution 1:5,000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293 and [6] NIH-3T3 cell lysates. A band at about 280kDa is full length intact MAP2A/2B isotypes while bands at about 70kDa represent MAP2C/D isotypes. Multiple bands in between are likely in vivo fragments of MAP2A/B. Only the SH-SY5Y cells, which have neuronal properties express MAP2 protein. The CPCA-Vim antibody binds to a single band at ~50kDa present in all preparations.
Mouse Monoclonal Antibody to MAP2A/B/C/D Cat# MCA-2C4
$120.00 – $800.00
Microtubules are 25nm diameter protein rods found in most eukaryotic cells and are associated with a family of proteins called microtubule associated proteins (MAPs). MAPs play a crucial role in the regulation of microtubule dynamics and interactions in vivo. MAP2 was originally named as one of the higher molecular weight MAPs with an SDS-PAGE molecular weight of about 280kDa (1-3). There is a single mammalian MAP2 gene which may generates two high molecular weight proteins of ~280kDa on SDS-PAGE named MAP2A and MAP2B and multiple lower molecular weight forms usually named MAP2C and MAP2D which run on SDS-PAGE gels at 60-70kDa. The lower molecular weight forms are found in neurons early in development, but as the animal matures they are replaced by the higher molecular weight forms (1,2). MAP2 isoforms are expressed only in neurons in perikarya and dendrites, so MAP2 antibodies are useful for identifying neurons in cell culture and sectioned material (4-8). MAP2C and D contain an “intrinsically unstructured region”, one of the prototypes for this widespread type of protein sequence (9). Since MAP2C and D are expressed earlier in development than MAP2A and B this antibody can be used for monitoring early neuronal development, though it is also useful as a general marker for neurons and dendrites in mature tissues.
This antibody was made against a recombinant full length form of human MAP2D and was found to bind all four MAP2 gene products meaning that it binds to the shared core region of these molecules. EnCor markets other mouse monoclonal antibodies specific for MAP2A and MAP2B MCA-4H5 and MCA-5H11. EnCor also markets MAP2A and MAP2B chicken and goat polyclonal antibodies CPCA-MAP2 and GPCA-MAP2. Mouse select image above left for larger view.
Chromogenic immunostaining of a formalin fixed paraffin embedded human hippocampus section with mouse mAb to MAP2A/B/C/D, MCA-2C4, dilution 1:5,000, detected in DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. In this image, the MAP2 antibody specifically labels neuronal cell bodies and projections. This antibody performs well in testing with both 4% PFA and standard NBF fixed tissues. Mouse select image for larger view.
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