EnCor Biotechnology
Mouse Monoclonal Antibody to Nsr1p Cat# MCA-31C4
Description
MCA-31C4 was isolated during a screen for monoclonal antibodies reactive with yeast nucleolar proteins. We determined the specificity by screening a yeast genomic library expressed as β-galactosidase fusion proteins in bacteriophage λgt11. Two individually isolated, different positive clones were found. Sequencing revealed that in both clones bound amino acids 190 and onwards in Nsr1p, the yeast homologue of nucleolin. MCA-31C4 therefore reacts with an epitope in the C-terminal 55% of Nsr1p. The antibody is provided as sterile-filtered cell culture fluid from an Integra CL-350 biochamber plus sodium azide.
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| Name: | Nsr1p, mouse monoclonal, Cat# MCA-31C4 |
| Immunogen: | Yeast nuclear preparation |
| UniProt: | G2WEN9 |
| Molecular Weight: | 44.5Da |
| Host: | Mouse |
| Isotype: | IgG1 |
| Species Cross-Reactivity: | Saccharomyces cerevissiae |
| RRID: | AB_2298370 |
| Format: | Concentrated hybridoma tissue culture media plus 5mM NaN3 |
| Applications: | WB, IF/ICC |
| Recommended Dilutions: | WB 1:500, IF/ICC 1:1,000 |
| Storage: | Store at 4°C for short term, for longer term at -20°C. Avoid freeze/thaw cycles. |
Nsr1p is localized in the nucleolus and binds to telomeric single-stranded oligonucleotide repeat sequence of the type and binds to the single-stranded telomeric repeat sequence (TG1-3)n. It has a role in ribosomal RNA maturation and possibly in transport of proteins into the nucleus.
This antibody has not been tested on formalin fixed and paraffin embedded samples for IHC, and so cannot be recommended for this purpose.
1. Lin JJ and Zakian VA. Isolation and characterization of two Saccharomyces cerevisiae genes that encode proteins that bind to (TG1-3)n single strand telomeric DNA in vitro. Nucleic Acids Res. 22:4906-13 (1994).
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