Aurora A/B kinase, mouse monoclonal, Cat# MCA-3H1

Aurora A/B kinase, mouse monoclonal, Cat# MCA-3H1

HeLa cell cultures were stained with MCA-3H1 antibody (green). The antibody stains spindle poles and mitotic spindles at anaphase and concentrates on the midbody between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Cells were counterstained with our chicken polyclonal antibody to Vimentin CPCA-Vim in red. Blue is a DNA stain.

Left: Western blot analysis of MCA-3H1 in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with MCA-3H1. Nocodazole is a microtubule depolymerizing agent which induces cells to halt at the G2/M phase and also induces Aurora A expression. The MCA-3H1 monoclonal binds strongly to a band at about 46 kDa, which is Aurora A and also shows binding to a band at 38 kDa. Right: Blots of recombinant human Aurora A, B and C were probed with MCA-3H1, which binds to both Aurora A and B. We conclude that the 38 kDa band seen in the HeLa extract is Aurora B. This is also consisted with the immunocytochemical staining of both centrosomes and midbodies seen on HeLa cells.

Product name Anti-Aurora A/B kinase
Description Mouse Monoclonal to Aurora A/B kinase.
Reference Code MCA-3H1
RRID# AB_2572230
Molecular weight 46 kDa, 38 kDa
Immunogen Full length recombinant human Aurora A protein expressed in and purified from E. coli.
Isotype IgG1
Concentration Antibody is supplied as an aliquot of 1 mg/mL of affinity purified antibody.
Species Reactivity Human, horse, cow, pig, chicken, rat, mouse
Applications Western blot, ICC/IF, IHC
Suggestions for use Western blots: 1:1,000.
ICC/IF or IHC: 1:500-1:1,000.

Aurora proteins are a family of serine/threonine protein kinases that play a key role in the regulation of cell division. The first Aurora kinase was discovered in Drosophila (1). Mutations of this kinase cause monopolar spindles surrounded by kinase, and the appearance of this was reminiscent of the Aurora borealis at the poles of the earth (1). Mammalian genomes encode 3 Aurora kinases named Aurora A, Aurora B, and Aurora C. All 3 contain a regulatory domain at the N terminus which is quite different between the molecules followed by a catalytic serine/threonine kinase domain which is almost identical between them.To download a sequence alignment of the 3 human Aurora proteins go here. As a consequence antibodies raised against one Aurora family member frequently cross-react with other family members. Since There is a short C-terminal peptide which is also variable between the three molecules (2). Aurora A is required for centrosome duplication, entry into mitosis, formation of bipolar spindlse and mitotic checkpoint (3). Aurora B is a chromosomal passenger protein and essential for chromosome condensation, kinetochore functions, spindle checkpoint activation and cytokinesis completion (4). Aurora C is heavily expressed in testis and is involved in spermatogenesis, but is also expressed in many cell lines and cancer cells and has been less well studied to date (5). Aurora A is first associated with centrosomes and then with spindle microtubules whereas Aurora B localizes to the spinal midzone and finally accumulates at the midbody. MCA-3H1 was raised against full length recombinant human Aurora A expressed in and purified from E. coli. The antibody was tested for binding to expressed human Aurora A, B and C and shown to react with both Aurora A and B, but not C (see Blot image). The HGNC names for Aurora A, B and C are AURKA, AURKB and AURKC. Since this antibody cross-reacted between Aurora A and B, we now have in progress monoclonal antibodies specific for only one Aurora gene product. The first of these is MCA-1A11, which is completely specific for Aurora A. MCA-3H1 is an excellent reagent for the visualization of mid bodies, centrosomes and spindles in dividing cells.


1. Glover DM, Leibowitz MH, McLean DA, Parry H. Mutations in aurora prevent centrosome separation leading to the formation of monopolar spindles. Cell 81:95-105 (1995).

2. Hochegger H, Hegarat N, Pereira-Leal JB. Aurora at the pole and equator: overlapping functions of Aurora kinases in the mitotic spindle. Open Biol. Mar 20;3(3):120185 (2013).

3. Barr AR, Gergely F. Aurora-A: the maker and breaker of spindle poles. J Cell Sci.120:2987-96 (2007).

4. Andrew PD, Knatko E, Moore WJ, Swedlow JR. Mitotic mechanics: the auroras come into view. Curr Opin Cell Biol.15(6):672-83 (2003).

5. Tang CJ, Lin CY, Tang TK. Dynamic localization and functional implications of Aurora-C kinase during male mouse meiosis. Dev Biol. Dev Biol. 290(2):398-410 (2006).

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