October 2018 News
We now have a catalog ready for downloading, select here. It’s a big catalog with a lot of high resolution images, so downloading may take some time. Also our founder was recently interviewed at the new Cade Museum for Creativity and Invention in Gainesville Florida. The interview was about the history of EnCor Biotechnology and this can be heard on various on line platforms including Buzzsprout, which can be accessed here.
September 2018 News
We release yet more antibodies! While we were upgrading our website quite a backlog built up, so we are releasing an unusual number of new reagents this month. We previously generated a rabbit polyclonal antibody to adenylate cyclase III (ACIII), RPCA-ACIII, which is an excellent marker of neuronal cilia in human and rodent cells and tissues. We now release two new reagents which are also good neuronal cilia markers, specifically mouse monoclonal antibody to ACIII MCA-1A12 and chicken polyclonal antibody to ACIII CPCA-ACIII. These were made against the same ACIII peptide as our original rabbit antibody, which has become widely used, have very similar properties and so will complement the rabbit antibody. We improved our western blotting and immunofluorescence data for our antibody to the product of the Ewing’s sarcoma breakpoint 1 EWSR1 gene, the EWS protein, antibody MCA-5H7. We also have additional data for our mouse monoclonals to methyl-CpG binding protein 2 (MeCP2), MCA-4F11 and MCA-5H12. There is a new antibody to FOX2, CPCA-FOX2 made in chicken, which specifically binds the nuclei of neurons many of which are not recognized by antibodies to NeuN, now known to correspond to a close relative of FOX2, namely FOX3. There is also a new rabbit polyclonal antibody to the serotonin transporter RPCA-SERT, a useful marker of serotinergic neurons and their thin beaded processes. A new rabbit polyclonal antibody to fibrillarin RPCA-Fib, an excellent marker of nucleoli to complement our widely used mouse and chicken antibodies to the same protein. Also new antibodies to myristoylated alanine rich C-kinase substrate (MARCKS), a chicken polyclonal CPCA-MARCKS and a mouse monoclonal MCA-5F9.
August 2018 News
We release two more new goat polyclonal antibodies to add to the neuronal perikarya and dendritic marker MAP2, GPCA-MAP2, namely to the mesenchymal and CNS intermediate filament protein vimentin, GPCA-Vim and to green fluorescent protein GPCA-GFP. Also mouse monoclonals to GFP MCA-3B11 and MCA-1F1, also a rabbit polyclonal antibody to the same protein RPCA-GFP. Also purified recombinant GFP PROT-r-AcGFP-GFP, the form of GFP expressed in a variety of widely used expression vectors. Also new antibodies to ankyrin 3, also known as ankyrinG, a protein expressed in the axonal initial segment and the nodes of Ranvier, made in mouse, rabbit and chicken, MCA-2A8, RPCA-ANK3 and CPCA-ANK3. Also new antibodies to fatty acid binding protein 7, also known as brain lipid binding protein, made in mouse MCA-2A84, and rabbit RPCA-FABP7. Also we add two new antibodies to tyrosine hydroxylase, an enzyme heavily expressed in all catecholaminergic neurons, MCA-4H2 and RPCA-TH, to add to the chicken antibody CPCA-TH we released in June. Finally another mouse monoclonal antibody to neurofilament subunit NF-L MCA-1B11, and a novel antibody to the neuronal stem cell marker Nestin, made in chicken CPCA-Nestin.
July 2018 News
We release a new antibody to MAP2A/B made in goat, useful for identifying neurons and their dendrites in a cell culture and sectioned material, GPCA-MAP2. Since this is made in goat it can be used on transgenic mice along with one or other of our rabbit or chicken antibodies for double or triple labeling of cells in culture or in sections. We also release a chicken polyclonal antibody to tyrosine hydroxylase (TH) CPCA-TH, which can be used to identify TH positive/chatecholaminergic neurons and processes in cell culture and sectioned material.
Science Daily is one of several on line journals which makes use of many of our images to illustrate their articles. For recent examples see one of our HeLa cell images, our astrocyte image here and here. Other frequent users of our images are Neuroscience News, Science News. Our current policy is to make our images freely available for any purpose, so contact us if you want to use a particular image you have seen on our web site, we have higher resolution versions of most of our images available.
April 2018 News
Since we spent a lot of time updating our website we did not get round to posting information about several new antibodies now available for shipping. There is new rabbit polyclonal antibody to galectin-3, RPCA-Gal3, an interesting multifunctional protein. There is a new chicken polyclonal antibody to secretagogin, a small Calcium binding protein expressed in certain subclasses of brain neuron CPCA-SCGN. There is also a novel antibody to DJ1, also known as PARK7 RPCA-DJ1. All antibodies are well characterized and we post images of western blots and stained cells and/or tissues.
March 2018 News
We finally get done with updating our web site, after several months of playing around with it, so we hope you like it!
In other news have made an excellent rabbit antibody to IBA1 (a.k.a. AIF1), RPCA-IBA1. The IBA1 protein is heavily expressed in microglia but not in neurons, astrocytes, oligodendrocytes or other kinds of CNS cell. As a result, antibodies to IBA1 are widely used to identify and study microglia in CNS cultures and sections. Microglia are the immune cells of the CNS have important roles in the cellular responses to a variety of CNS damage and disease states. We also have an excellent high resolution 24″ X 36″ poster showing staining of microglial cells with this antibody on rat cerebellum, poster-27. Here is an image of that.
We continue to characterize our antibodies in ever greater detail; for example we recently epitope mapped our two monoclonal antibodies to the intermediate filament protein vimentin, MCA-2A52 and MCA-2D1. This allows us to confidently predict which species these antibodies will work on. For another example see our new findings made on the widely used mouse monoclonal antibody to neurofilament NF-L MCA-DA2.
