| Name: | Goat Polyclonal Antibody to GFP |
| Immunogen: | Recombinant AcGFP protein purified from E. coli |
| HGNC Name: | NA |
| UniProt: | Q6YGZ0 |
| Molecular Weight: | ~27kDa |
| Host: | Goat |
| Isotype: | |
| Species Cross-Reactivity: | NA |
| RRID: | AB_2737371 |
| Format: | Affinity purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3 |
| Applications: | WB, IF/ICC, IHC |
| Recommended Dilutions: | WB: 1:1,000-5,000 IF/IHC: 1:5,000 |
| Storage: | Store at 4°C for short term, for longer term at -20°C |
Immunofluorescent analysis of transfected HEK293 cells with a GFP-construct in green stained with goat pAb to GFP, GPCA-GFP, dilution 1:5,000 in red. The blue is Hoechst staining of nuclear DNA. The GPCA-GFP antibody reveals GFP protein expressed only in transfected cells, as a result transfected cells are appeared express both red and green signals and so appear an orange-golden color. Untransfected cells show neither signal.
Western blot analysis of HEK293 cell lysates using goat pAb to GFP, GPCA-GFP, dilution 1:1,000, in green: [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct and [4] cells transfected with an mCherry construct. Strong band at ~27kDa corresponds to GFP protein detected only in cells transfected with GFP construct. This antibody does not recognize the mCherry protein.
Goat Polyclonal Antibody to GFP
Cat# GPCA-GFP
$150.00 – $1,000.00
The green fluorescent protein (GFP) is a 27kDa protein isolated originally from the jellyfish Aequoria victoria. It has an endogenous fluorochrome activity with excitation maximum at 395nm and emission maximum at 509nm, which is similar to that of fluorescein (1,2). The GFP gene was cloned and sequenced and the origin of the fluorochrome by autocatalytic activity of certain amino acids was discovered (3,4). Much interest in GFP was generated when it was shown that fluorescence develops rapidly when the protein is expressed and requires only molecular oxygen and no other cofactors. As a result GFP can be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell (5). GFP has been engineered to produce a vast number of variously colored mutants including blue, cyan and yellow protein derivatives, BFP, CFP and YFP (6-9). GFP and other fluorescent proteins derived from jellyfish, coral and other Cnidaria are widely used as tracers in transfection and transgenic experiments to monitor gene expression and protein localization in vivo and in in vitro. The crystal structure of GFP was determined (7) which allowed amino acid modifications to improve spectral properties and prevent multimerization (8,9). The 2008 Nobel prize in chemistry was awarded “for the discovery and development of the green fluorescent protein, GFP”.
The GPCA-GFP antibody was made against a recombinant GFP construct originating from an Aequoria species which was engineered to improve spectral properties and prevent oligomerization (10). This form of GFP, referred to as AcGFP, is 94% identical to the eGFP developed by Tsien and coworkers and is the form of GFP inserted in the Clontech/Takara pAcGFP and related expression vectors. We also supply the immunogen, PROT-r-AcGFP. The antibody can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals in tissues of transgenic animals. We also supply mouse monoclonal antibodies and rabbit and chicken polyclonal antibodies to this protein, MCA-3B11, MCA-1F1, RPCA-GFP and CPCA-GFP. Mouse select image above left for larger view.
Chromogenic immunostaining of formalin fixed paraffin embedded GFP transduced mouse brain with goat pAb to GFP, GPCA-GFP, dilution 1:5,000, detected with DAB (brown) using the Vector Elite ABC-HRP detection and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. GPCA-GFP specifically detected GFP positive cells in the cerebellum as expected for this model. Mouse select on image for larger view.
We used AcGFP used as immunogen for this antibody. The sequence was. taken from AY233272, download here, also Uniprot entry Q6YGZ0
1 MVSKGAELFT GIVPILIELN GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT
61 LVTTLSYGVQ CFSRYPDHMK QHDFFKSAMP EGYIQERTIF FEDDGNYKSR AEVKFEGDTL
121 VNRIELTGTD FKEDGNILGN KMEYNYNAHN VYIMTDKAKN GIKVNFKIRH NIEDGSVQLA
181 DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMI YFGFVTAAAI THGMDELYK
This construct is available as PROT-r-AcGFP
Above, mice transgenic for green fluorescent protein (GFP) under the Thy1 promoter express GFP in cerebral pyramidal cells. The image is of a 400μM maximum intensity optical section taken from an entire Thy1-GFP mouse brain “cleared” in the Lifecanvas lab. Lifecanvas scientists then stained the brain with EnCor goat polyclonal antibody to GFP GPCA-GFP coupled to a red dye. As expected, the green and red signals superimpose in the perikarya of the pyramidal cells which appear yellowish. The antibody also stains the apical dendrites which have a lower level of GFP expression, effectively amplifying the GFP signal. Mouse select on image for larger view.
1. Shimomura O, Johnson FH, Saiga Y. Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J. Cell. Comp. Physiol. 3:223–39 (1962).
2. Shimomura, O. Structure of the chromophore of Aequorea green fluorescent protein. FEBS Lett. 104:220–2 (1979).
3. Prasher DC, et al. Primary structure of the Aequorea victoria green-fluorescent protein. Gene 111:229-33 (1992).
4. Cody CW, et al. Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein. Biochem. 32:1212-8 (1993).
5. Chalfie M, et al. Green Fluorescent protein as a marker for gene expression. Science 263:802-5 (1994).
6. Heim R, Prasher DC, Tsien RY. Wavelength mutations and post-translational autoxidation of green fluorescent protein. PNAS 91:12501-04 (1994).
7. Ormo M, et al. Crystal structure of the Aequorea victoria green fluorescent protein. Science 273:1392-95 (1996).
8. Tsien RY. The green fluorescent protein. Annu. Rev. Biochem. 67:509-44 (1998).
9. Zacharias DA, Violin JD, Newton AC, Tsien RY. Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science 296:913-6 (2002).
10. Gurskaya NG, et al. A colourless green fluorescent protein homologue from the non-fluorescent hydromedusa Aequorea coerulescens and its fluorescent mutants. Biochem. J. 373:403-8 (2003).
