Immunohistological analysis of brain stem section stained with rabbit pAb to laminin, RPCA-Laminin, dilution 1:1,000 in red, and costained with chicken pAb to myelin basic protein (MBP), <a href="http://encorbio.com/products/cpca-mbp/" target="_blank" rel="noopener">CPCA-MBP</a>, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were sta
Immunohistological analysis of brain stem section stained with rabbit pAb to laminin, RPCA-Laminin, dilution 1:1,000 in red, and costained with chicken pAb to myelin basic protein (MBP), <a href="http://encorbio.com/products/cpca-mbp/" target="_blank" rel="noopener">CPCA-MBP</a>, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were sta

EnCor Biotechnology

Rabbit Polyclonal Antibody to Laminin Cat# RPCA-Laminin

$300.00
Description

Laminin 111
Rabbit Polyclonal Antibody

 Collections:
Amount: 100µL of 1mg/mL
Amount: 100µL of 1mg/mL
Immunohistological analysis of brain stem section stained with rabbit pAb to laminin, RPCA-Laminin, dilution 1:1,000 in red, and costained with chicken pAb to myelin basic protein (MBP), CPCA-MBP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies. The laminin antibody is an excellent marker of basement membranes surrounding blood vessels, while the MBP antibody stains the myelin sheathes around axons.
Western blot analysis of different tissue lysates using rabbit pAb to laminin, RPCA-Laminin, dilution 1:1,000 in green: [1] protein standard (red) and rat tissue lysates: [2] retina, [3] brain, [4] lung, [5] pancreas, [6] spleen. Mouse tissue lysates: [7] retina, [8] brain, [9] lung, [10] pancreas, and [11] spleen. The strong band around 250kDa mark, corresponds to laminin protein. Smaller proteolytic fragments of laminin are also detected with this antibody.

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Name: Laminin 111, rabbit polyclonal, Cat# RPCA-Laminin
Immunogen: Laminin 111 from mouse EHS sarcoma cells obtained from Sigma-Aldrich,
HGNC Name: LAMA1, LAMB1, LAMC1
UniProt: P25391, P07942, P11047
Molecular Weight: 440kDa, 220kDa, 158kDa
Host: Rabbit
Isotype: IgG
Species Cross-Reactivity: Human, Rat, Mouse
RRID: AB_2572341
Format: Affinity purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Applications: WB, IF/ICC, IHC
Recommended Dilutions: WB 1:5,000. IF/ICC and IHC: 1:1,000-5,000.
Storage: Storage for short term at 4°C recommended, for longer term at -20°C.



1. Timpl R, et al. Laminin--a glycoprotein from basement membranes. J. Biol. Chem. 254:9933-7 (1979).


2. Kleinman HK, et al. Isolation and Characterization of Type IV Procollagen, Laminin, and Heparan Sulfate Proteoglycan from the EHS Sarcoma. Biochemistry 21:6188-93 (1982).


3. Colognato H, Yurchenco PD. Form and function: the laminin family of heterotrimers.Dev. Dyn. 218:213-34 (2000).


4. Durbeej M, Laminins. Cell Tiss. Res. 339:259-268 (2010).


5. Burgeson RE, et al. A new nomenclature for the laminins. Matrix Biology 14:209-11 (1994).


6. Aumailley M, et al. A simplified laminin nomenclature. Matrix Biology 24:326-332 (2005).


7. Ekblom M, et al. Laminin isoforms and epithelial development. Ann. NY Acad. Sci. 857:194-211 (1998).


8. Alpy F, et al. Generation of a conditionally null allele of the laminin alpha1 gene. Genesis 43:59-70 (2005).


9. Rooney JE, Gurpur PB, Burkin DJ. Laminin-111 protein therapy prevents muscle disease in the mdx mouse model for Duchenne muscular dystrophy. PNAS 106:7991-6 (2009).


10. Gawlik KI, Oliveira BM, Durbeej M. Transgenic Expression of Laminin α1 Chain Does Not Prevent Muscle Disease in the mdx Mouse Model for Duchenne Muscular Dystrophy. Am. J. Pathol. 178:1728-37 (2011).

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