Rabbit Polyclonal Antibody to Coronin 1a Cat# RPCA-Cor1a

      Coronin was originally discovered in the slime mold Dictyostelium, where it was found to be involved in the chemotactic response of these amoeboid cells (1,2). The name derives from from "corona", which is Latin for crown, since the protein was localized at the leading edge or crown of these highly motile cells. In mammals, there are at least five coronin family proteins derived from 5 distinct genes. These are named coronins 1 to 5 in one nomenclature or coronins 1a and 1b, 2a, 2b and 2c in another (1,2). Coronins are members of the WD or WD40 family of proteins, which are generally involved in mediating important protein to protein interactions (3). The basic structure of WD molecules is a seven bladed β-propellor structure each blade being based on some variant of a ~40 amino acid WD (Trp-Asp) containing peptide. Structural studies show that coronin 1a is a typical 7 bladed β-propellor member of this protein family (4) Coronin 1a, a.k.a coronin 1 is found exclusively in hematopoietic lineage cells such as lymphocytes, macrophages and neutrophils. The only hematopoietic cells normally found within the neuropil and white matter of the central nervous system are the microglia, so antibodies to coronin 1a are useful to identify this important cell type (5). Microglia mediate immune responses in the CNS and are able to actively phagocytose infectious or damaged material. They are very active cells, constantly migrating through the brain and interacting with other cells (6). A typical response to CNS damage or disease is increase the number of microglia at the compromised site.<

Chromogenic immunostaining of a formalin fixed paraffin embedded human brain cortex section with rabbit pAb to coronin 1a, RPCA-Cor1a, dilution 1:2,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. The RPCA-Cor1a antibody specifically labels the perikarya and processes of microglial cells. This antibody performs well in testing with 4% PFA and standard NBF fixed mouse, rat, and human tissue. Mouse select image for larger view.

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