A recent very impressive paper in Science Advances describes a detailed 3D map of the entire mouse brain using triple antibody labelling with a collection of cell type and cell structure specific antibodies, this data being correlated with high resolution MRI. The authors impregnated the brains fully with the antibodies and imaged them with light sheet microscopy using procedures developed by LifeCanvas. The authors made use of three of EnCor's mouse antibodies, our FOX3/NeuN MCA-1B7, our myelin basic protein antibody MCA-7G7 and one of our our NF-H antibodies, apparently MCA-NAP4.
We have generated a novel rabbit polyclonal antibody to neuronal nitric oxide synthase (nNOS, NOS1), RPCA-NOS1. NOS1 is a brain enzyme which reacts with the amino acid arginine to generate the important signaling molecule and gas nitric oxide (NO). NO can rapidly diffuse through tissues as it easily passes cytoplasm and cellular membranes. NO activates guanylate cyclase which generates cyclic GMP which in turn activates many other molecules. NO is involved in controlling blood vessel diameter and hence blood flow and pressure in the CNS. NO also has rolls in memory formation, the regulation of synaptic transmission and S-nitrosylation of proteins. Our antibody was made against the C-terminal peptide of human nNOS and we document that it works well on human, rat and mouse tissues for western blotting, IF, ICC and IHC.
A new collaborative paper between EnCor scientists and the University of Florida just appeared in the prestigious and rigorously peer-reviewed journal Experimental Neurology. The paper making use of one of our novel Degenotag™ reagents, specifically our epitope mapped NF-L monoclonal MCA-6H63, which detects forms of NF-L only accessible after neurodegeneration. See Fusco et al. "Serum evaluation of NFL correlates with histological identification of degenerating axons", Exp. Neurol. 23:392:115360 (2025). Finally our CEO and founder, Gerry Shaw, gave a presentation on this and some of our other recent research at the 5th International Research Conference on Neurodegenerative Disease, IRCND2025.
