| Name: | Mouse monoclonal antibody to alpha-Internexin |
| Immunogen: | Full length recombinant rat α-internexin expressed in and purified from E. coli. |
| HGNC Name: | INA |
| UniProt: | P23565 |
| Molecular Weight: | 64-66 kDa by SDS-PAGE |
| Host: | Mouse |
| Isotype: | IgG1 |
| Species Cross-Reactivity: | Human, Cat, Rat, Mouse |
| RRID: | AB_2572334 |
| Format: | Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3 |
| Applications: | WB, IF/ICC, IHC |
| Recommended Dilutions: | WB: 1:10,000. IF/ICC and IHC: 1:5,000. |
| Storage: | Store at 4°C for short term, for longer term at -20°C. |
Immunofluorescent analysis of rat cerebellum section stained with mouse mAb to α-internexin, MCA-1D2, dilution 1:5,000 in green, and costained with chicken pAb to calretinin, CPCA-Calret, 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies. The α-internexin antibody selectively stains neuronal processes, in particular parallel fibers, the axons of granule cells. Calretinin antibody stains interneurons predominantly in the molecular layer of the cerebellum.
Western blot analysis of different tissue lysates using mouse mAb to α-internexin, MCA-1D2, dilution 1:10,000 in green: [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig spinal cord and [7] cow spinal cord. MCA-1D2 antibody reveals the α-internexin protein with apparent molecular weight of 64 to 66 kDa with slight variability among species.
Mouse Monoclonal Antibody to α-Internexin
Cat# MCA-1D2
$120.00 – $800.00
α-internexin is a Class IV intermediate filament protein originally discovered by two different groups of researchers as it copurifies with NF-L, NF-M and NF-H, the better known major neurofilament “triplet” subunits (1,2). It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Some neurons express α-internexin in the absence of NF-L, NF-M and NF-H, though most mature neurons express all four proteins. α-internexin antibody has been shown, in peer reviewed publications, to reveal the upregulation of α-internexin in facial neurons following experimental axotomy followed by down regulation on axonal regeneration (3). It has also been used to identify and classify patients with neurofilament inclusion body disease, a specific form of frontotemporal lobar dementia (4-6). Finally it can be used to confirm the presence of circulating antibodies to α-internexin in the blood of certain patients with endocrine autoimmunity (7).
This antibody was made against full length recombinant rat α-internexin, and the antibody binds to the α-internexin protein from different mammals, including human, rat, and mouse. It is clean and specific on western blots, ICC and IHC. We also supply an alternate mouse monoclonal antibody, MCA-2E3, a rabbit polyclonal antibody, RPCA-a-Int, and a chicken polyclonal antibody, CPCA-a-Int, to this protein. Mouse select image at left for larger view.
Chromogenic immunostaining of a 4% PFA fixed paraffin embedded rat cerebellum section with mouse mAb to α-internexin, MCA-1D2, dilution 1:1,000, detected in DAB (brown) using ImmPress method with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. The 1D2 α-internexin antibody labels parallel fibers and other neuronal processes in both rat and human brain. This antibody performs well in testing with both 4% PFA and standard NBF fixed tissues but does not stain long term NBF fixed tissue effectively. We therefore recommend our alternate mouse monoclonal antibody to α-internexin MCA-2E3 for such paraffin applications. Mouse select image for larger view.
1. Pachter J and Liem RKH. Alpha-Internexin, a 66-kD intermediate filament-binding protein from mammalian central nervous tissues. J Cell Biol 101:1316-22 (1985).
2. Chiu FC, et al. Characterization of a novel 66 kd subunit of mammalian neurofilaments. Neuron 2:1435-45 (1989).
3. McGraw T. et al. Axonally transported peripheral signals regulate alpha-internexin expression in regenerating motoneurons. J Neurosci. 22:4955-63 (2002).
4. Evans J. et al. Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem. J. Neurophysiol. 87:1076-85 (2002).
5. Cairns NJ. et al. Alpha-internexin is present in the pathological inclusions of neuronal intermediate filament inclusion disease. Am . J. Pathol. 164:2153-61 (2004).
6. Uchikado H1, Shaw G, Wang DS, Dickson DW.
Screening for neurofilament inclusion disease using alpha-internexin immunohistochemistry. Neurology 64:1658-9 (2005).
7. Rajasalu T, et al. Demonstration of natural autoantibodies against the neurofilament protein alpha-internexin in sera of patients with endocrine autoimmunity and healthy individuals. Immunol. Lett. 94:153-60 (2004).
