| Name: | Mouse Monoclonal Antibody to Aurora B kinase |
| Immunogen: | Full length human recombinant aurora B protein expressed in and purified from E. Coli |
| HGNC Name: | AURKB |
| UniProt: | Q96GD4 |
| Molecular Weight: | 38kDa |
| Host: | Mouse |
| Isotype: | IgG1 |
| Species Cross-Reactivity: | Human, Horse, Dog |
| RRID: | AB_2572234 |
| Format: | Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3 |
| Applications: | WB, IF/ICC, IHC |
| Recommended Dilutions: | WB: 1:1,000. IF/ICC or IHC: 1:1,000-1:2,000. |
| Storage: | Store at 4°C for short term, for longer term at -20°C |
Immunofluorescent analysis of HeLa cells stained with mouse mAb to aurora B kinase, MCA-6G2, dilution 1:1,000 in red, and costained with chicken pAb to vimentin, CPCA-Vim, dilution 1:10,000 in green. Blue is DAPI staining of nuclear DNA. MCA-6G2 antibody produces strong staining associated with chromosomes in prophase (A), the centromere in prometaphase and metaphase (B), the central mitotic spindle in anaphase (C), and midbodies between the two daughter cells during telophase and beyond (D). The vimentin antibody stains the intermediate filament network in these cells.
Western blot analysis of different cell lysates and recombinant protein solutions using mouse mAb to aurora B, MCA-6G2. Left: cells were treated with 100ng/mL of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase. [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] equine NBL6 cells, and [5] mouse KR158 cells. Right: Human recombinant proteins aurora A, B, and C as indicated. This antibody binds specifically to aurora B.
Mouse Monoclonal Antibody to Aurora B kinase
Cat# MCA-6G2
$120.00 – $800.00
Aurora proteins are a family of serine/threonine protein kinases which play a key role in the regulation of cell division which were originally discovered in studies of Drosophila (1). Mammalian genomes encode 3 aurora kinases named aurora A, B and C, each containing a variable regulatory domain at the N terminus followed by a catalytic serine/threonine kinase domain which is almost identical between them, see here for sequence alignment. As a result it is possible to generate antibodies which react with only one aurora kinase or cross react with two or more other kinases. Aurora A and B are almost ubiquitous in distribution while C is normally only expressed in testis. Aurora A is required for centrosome duplication, entry into mitosis, formation of bipolar spindle and mitotic checkpoint (3). Aurora B is a chromosomal passenger protein and essential for chromosome condensation, kinetochore functions, spindle checkpoint activation and cytokinesis completion (4). Aurora C is normally involved in spermatogenesis, but may also be expressed in many transformed cell lines and tumors and has been less well studied to date (5). The aurora kinases are essential for the progression to cell division and as a result there has been much interest in the development of drugs aimed at inhibiting their activity for use as anticancer agents (6,7). We have made a panel of antibodies to the aurora kinases, concentrating originally on aurora A and B, and we made recombinant full length human aurora constructs of all three to document their potential cross reactivity.
The MCA-6G2 antibody was made against full length human aurora B protein and was shown to bind only aurora B, but not aurora A or C. The antibody can be used to identify dividing or soon to be dividing cells since it binds to the inner centromere and is also an excellent marker of midbodies both during and after cell division. We also supply other aurora specific antibodies, to aurora A MCA-1A11, antibodies to both aurora A and B, MCA-5A12 and MCA-6G2, and another which is aurora B specific, MCA-3F11. Mouse select image at left for larger view.
A sequence alignment of the 3 human aurora molecules can be downloaded from here.
1. Glover DM, Leibowitz MH, McLean DA, Parry H. Mutations in aurora prevent centrosome separation leading to the formation of monopolar spindles. Cell 81:95-105 (1995).
2. Hochegger H, Hegarat N, Pereira-Leal JB. Aurora at the pole and equator: overlapping functions of Aurora kinases in the mitotic spindle. Open Biol. 20:120185 (2013).
3. Barr AR, Gergely F. Aurora-A: the maker and breaker of spindle poles. J. Cell Sci. 120:2987-96 (2007).
4. Andrew PD, Knatko E, Moore WJ, Swedlow JR. Mitotic mechanics: the auroras come into view. Curr. Opin. Cell Biol. 15:672-83 (2003).
5. Tang CJ, Lin CY, Tang TK. Dynamic localization and functional implications of Aurora-C kinase during male mouse meiosis. Dev. Biol. 290:398-410 (2006).
6. Andrews PD. Aurora kinases: shining lights on the therapeutic horizon? Oncogene (2005) 24:5005–15 (2005).
7. Boris AC, Bhatt HG. A comprehensive review on Aurora kinase: Small molecule inhibitors and clinical trial studies. Eur. J. Med. Chem. 140:1-19 (2017).
A sequence alignment of the 3 human aurora molecules can be downloaded from https://encorbio.com/Alignments/Aurora_alignment.pdf.
