| Name: | Rabbit polyclonal antibody to alpha-internexin |
| Immunogen: | Full length recombinant rat α-internexin expressed in and purified from E. coli. |
| HGNC Name: | INA |
| UniProt: | Q16352 |
| Molecular Weight: | 64-66 kDa by SDS-PAGE |
| Host: | Rabbit |
| Isotype: | |
| Species Cross-Reactivity: | Human, rat, mouse, cow, pig, horse |
| RRID: | AB_2572336 |
| Format: | Antibody is supplied as an aliquot of serum plus 5mM NaN3 |
| Applications: | Western blot, ICC/IF, IHC |
| Recommended Dilutions: | Western blot: 1:10,000-1:20,000. ICC/IF and IHC: 1:500-1:1,000. |
| Storage: | Store at 4°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles. |
Immunofluorescent analysis of rat cerebellum section stained with rabbit pAb to α-internexin, RPCA-a-Int, dilution 1:2,000, in green, and chicken pAb to GFAP, CPCA-GFAP, dilution 1:5,000, in red. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. The α-internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells and parallel fibers the axons of granule cells. The GFAP antibody labels network of glial cells, such as astrocytes in the granule cell layer and white matter and Bergmann glia in the molecular layer.
Western blot analysis of whole tissue lysates using rabbit pAb to α-internexin, RPCA-a-Int, dilution 1:10,000 in green: [1] protein standard (red), [2] mouse spinal cord, [3] rat spinal cord, [4] bovine spinal cord. Major bands in the 64-66kDa range corresponds to α-internexin. The α-internexin protein from different species is known to vary slightly in SDS-PAGE molecular weight.
Rabbit Polyclonal Antibody to α-Internexin/NF66
Cat# RPCA-a-Int
$120.00 – $800.00
α-internexin is a Class IV intermediate filament protein originally discovered by two different groups of researchers as it copurifies with NF-L, NF-M and NF-H, the then better known major neurofilament “triplet” subunits (1,2). It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Some neurons express α-internexin in the absence of NF-L, NF-M and NF-H, though most mature neurons express all four proteins. An α-internexin antibody has been shown, in peer reviewed publications, to reveal the upregulation of α-internexin in facial neurons following experimental axotomy followed by down regulation on axonal regeneration (3). The MCA-2E3 mouse monoclonal antibody to α-internexin, also made by EnCor is the standard reagent used to identify and classify patients with neurofilament inclusion body disease, a specific form of frontotemporal lobar dementia (4-6).
This antibody was made against full length recombinant rat α-internexin fused to the C-terminus of bacterial TrpE expressed in and purified from E. coli. The antibody binds to the α-internexin protein from different mammals, including human, rat, and mouse. It is clean and specific on western blots, ICC and IHC. We also supply an mouse monoclonal antibodies and a chicken polyclonal antibody to this protein, MCA-2E3, MCA-1D2 and CPCA-a-Int. Mouse select image at left for larger view.
Chromogenic Immunostaining of a formalin fixed paraffin embedded human cerebellum section with rabbit pAb to α internexin, RPCA-a-Int, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. The RPCA-a-Int antibody labels axons and dendrites of neuronal cells, outlining the Purkinje cells and parallel fibers in this image. This antibody performs well in testing with both 4% PFA and standard NBF fixed rat, mouse, and human tissues. Mouse select image for larger view.
1. Pachter J and Liem RKH. Alpha-Internexin, a 66-kD intermediate filament-binding protein from mammalian central nervous tissues. J Cell Biol 101:1316-22 (1985).
2. Chiu FC, et al. Characterization of a novel 66 kd subunit of mammalian neurofilaments. Neuron 2:1435-45 (1989).
3. McGraw T. et al. Axonally transported peripheral signals regulate alpha-internexin expression in regenerating motoneurons. J Neurosci. 22:4955-63 (2002).
4. Evans J. et al. Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem. J. Neurophysiol. 87:1076-85 (2002).
5. Cairns NJ. et al. Alpha-internexin is present in the pathological inclusions of neuronal intermediate filament inclusion disease. Am . J. Pathol. 164:2153-61 (2004).
6. Uchikado H1, Shaw G, Wang DS, Dickson DW. Screening for neurofilament inclusion disease using alpha-internexin immunohistochemistry. Neurology 64:1658-9 (2005).
