Rabbit Polyclonal Antibody to Catalase RPCA-Catalase

      Catalase is an enzyme found in all living organisms, including animals, plants, and microorganisms. In eukaryotes it is localized in peroxisomes, which are small, membrane-bound organelles responsible for various metabolic reactions in cells. Catalase catalyses the breakdown of hydrogen peroxide (H2O2) into water (H2O) and oxygen (O2). Hydrogen peroxide is a byproduct of various biochemical reactions in cells and can be harmful if allowed to accumulate, as it can modify and damage cellular proteins, lipids and nucleic acids. Catalase therefore prevents oxidative stress and maintains cellular health. Catalase forms a tetramer in vivo, and, like hemoglobin and the cytochromes, each subunit contains an iron containing heme group. The enzyme catalase is widely studied in biochemistry as liver is a rich source of the native protein which allowed purification, amino acid sequence determination and crystallization in the 60s and 70s. Catalase activity is important for the regulation of cellular physiology and changes in aging and diseases associated with oxidative stress.

Chromogenic immunostaining of a neutral buffered formalin fixed paraffin embedded rat kidney section with rabbit pAb to catalase, RPCA-Catalase, dilution 1:2,000, detected with DAB (brown) using the Vector Elite ABC-HRP detection and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. The RPCA-Catalase antibody strongly labels the peroxisomes in the cytoplasm of kidney tubule cells. This antibody performs well in testing with 4% paraformaldehyde and standard neutral buffered formalin fixed mouse, rat, and human tissue. Mouse select image for larger view.

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