Transfected HEK293 cells which overexpress GFP protein were stained with MCA-1F1 at 1: 2,000. Cells which are transfected with GFP are bright green in left panel. Staining with MCA-1F1 is shown in red in middle panel and this overlaps GFP expression so cells appear orange. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA.
|Blot of crude homogenate from HEK293 cells transfected with pFin-EF1-GFP vector (lane1) and non-transfected HEK293 (lane2) was probed with our MCA-1F1 antibody. The pFin-EF1-GFP vector is from the laboratory of Dr Susan Semple-Rowland, University of Florida, and expresses full length GFP. There is a strong clean band at about 27kDa in transfected cells corresponding to GFP which is absent from non-transfected cells.
||Chicken Polyclonal Antibody to GFP
||Prot-aceGFP recombinant protein
||Antibody is affitiny purified at 1mg/ml.
||Western blot, ICC/IF, IHC
||Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.
|Suggestions for use
||Western blot: 1:1,000-5,000
Green Fluorescent Protein (GFP) is a ~27 kDa protein isolated originally from the jellyfish Aequoria victoria. It is a fluorescent protein with an excitation maximum at 395 nm and emission maximum at 509 nm, so that when excited with blue or UV light, it emits green light (1). The fluorescence of GFP requires only the polypeptide chain and molecular oxygen and no other cofactors, so it can be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell. GFP has been engineered to produce a vast number of variously colored mutants including blue, cyan and yellow protein derivatives, such as BFP, CFP and YFP etc. (2-4). GFP and these derivatives are widely used as fluorescent tracers in transfection and transgenic experiments to monitor gene expression and protein localization in Vivo. GFP was the basis of the 2008 Nobel Prize in Chemistry, awarded to Osamu Shimomura, Martin Chalfie and Roger Tsien, specifically “for the discovery and development of the green fluorescent protein, GFP”.
1. Shimomura O, Johnson FH, Saiga Y. Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. Journal of Cellular and Comparative Physiology 3:223–39 (1962).
2. Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ. Crystal structure of the Aequorea victoria green fluorescent protein. Science 273:1392-95 (1996).
3. Heim R, Prasher DC, Tsien RY. Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc. Natl. Acad. Sci. USA 91:12501-04 (1994).
4. Lelimousin M, Noirclerc-Savoye M, Lazareno-Saez C, Paetzold B, Le Vot S, Chazal R et al. Intrinsic dynamics in ECFP and Cerulean control fluorescence quantum yield. Biochemistry 48:10038–10046.
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