December 2019 News
We have added more quantitative data about our various monoclonal antibodies, including several of the most widely used including our mouse monoclonals to fibrillarin (MCA-38F3 and MCA-4A4), also HSP60, (MCA-1C7), TDP43 (MCA-3H8) and several others. We also have new data on our widely used neurofilament NF-L antibodies (MCA-DA2 and MCA-7D1) and also phosphorylated neurofilament H (MCA-NAP4, MCA-AH1 and MCA-9B12).October 2019 News
We are going to the 2019 Society for Neuroscience meeting in Chicago, so come by Booth 468 to get some pens, flashlights, posters and other free stuffs! We also continue to improve the characterization of our antibodies, so we now know both the epitopes and the kinetic properties of an increasing number, for example see the latest data on our popular mouse monoclonals to FOX3/NeuN and myelin basic protein, MCA-1B7 and MCA-7D2, also to cFOS MCA-2H2 . We also release a new mouse monoclonal to neurofilament NF-L MCA-6H112 directed against the C-terminal peptide of this major structural protein. This antibody works well on blots, cell culture, sectioned material and in CLARITY and related tissue clearing methods, revealing neurons and their axons, and can also be used to monitor neurofilament proteolysis.
August 2019 News
We add new high quality antibodies to the extreme C-terminal peptide of neurofilament NF-L, a rabbit polyclonal RPCA-NF-L-ct. This antibody was made against the extreme C-terminal peptide of rat NF-L coupled to KLH, so the epitopic region is known. This rabbit antibody binds to all mammalian NF-L molecules tested to date. After we epitope mapped our monoclonal antibodies to FOX3/NeuN we found that all of them, and also antibodies made by other companies including the original NeuN monoclonal antibody A66, bind to the same proline rich peptide at the N-terminus of the molecule (see here). We therefore made a new version of our rabbit FOX3 antibody, RPCA-FOX3, raised against this N-terminal peptide. This antibody works well for cell staining and has the advantage over other polyclonal antibodies that the exact epitopic region is known. The peptide used is 100% conserved in most mammalian and avian species so the antibody will have wide utility. Finally we release a new epitope mapped mouse monoclonal antibody to mCherry, MCA-5A6. For epitope information on this antibody see here.
April 2019 News
We add a novel chicken polyclonal antibody which recognizes the neuron specific enolase (NSE), a.k.a. enolase 2 or γ enolase, CPCA-NSE. This recognizes NSE cleanly on western blots and in immunofluorescense experiments. This antibody is a complement to our rabbit polyclonal antibody to NSE, RPCA-NSE and our epitope mapped and highly specific mouse monoclonal antibody to the related protein enolase 1, also known as non-neuronal enolase or α enolase MCA-253.
March 2019 News
We continue with our program of epitope mapping our most widely used mouse monoclonal antibodies. We now add epitope information to the data sheet for our widely used monoclonal antibody to ubiquitin, MCA-Ubi-1. The epitope for this antibody maps to a peptide which is conserved in the ubiquitin molecule expressed in every important model organism from macaque to yeast, see here. We also mapped the epitopes for our excellent HSP60 MCA-1C7 and myelin basic protein MCA-7G7 antibodies and we provide more information about another myelin basic protein monoclonal with an epitope distinct from MCA-7G7, MCA-7D2. We also mapped the epitopes for our two mouse monoclonal antibodies to GFP, MCA-1F1 and MCA-3B11, to the N-terminus of the molecule. We also show that both antibodies recognize the two most widely used GFP proteins, AcGFP and eGFP. Finally we epitope mapped our two MAP tau mouse monoclonal antibodies, MCA-2E9. and MCA-5B10, to two distinct epitopes towards the C-terminus of human MAP tau molecules. The epitopes are expressed in all forms of tau and so both antibodies will recognize all tau isoforms on western blots and in IHC. The amino acid sequences including the epitopes are extremely conserved so the two antibodies will be usable in studies of essentially all mammals and many other vertebrates.
January 2019 News
Happy New Year! We continue to develop an ever better characterization of our antibodies and to add new reagents to our portfolio. For example we mapped the epitopes of our widely used antibodies to fibrillarin, MCA-38F3 and MCA-4A4, to two different peptides in the highly conserved fibrillarin domain of the human molecule, see here. We mapped the epitope of our versatile antibody to TDP43, MCA-3H8 to a highly conserved peptide in the C-terminal glycine rich region of this molecule, see here. We also mapped the epitope of our widely used antibody to FOX3/NeuN, MCA-1B7 to the N-terminal of this molecule to the same region as the original NeuN monoclonal antibody see here. This data significantly increases the utility of these antibodies, and epitope mapping of many other of our monoclonal antibodies is in progress and data will be posted on line shortly.
