We add recombinant forms of three of our most popular mouse antibodies, MCA-1B7 to Fox3/NeuN, MCA-4H2 to tyrosine hydroxylase and MCA-DA2 to the neurofilament light chain NF-L. These three reagents are very well characterized and have been used in many peer reviewed publications. However many leading journals now prefer scientists to use recombinant forms of monoclonal antibodies if they are available, so we cloned and sequenced these three clones allowing us to generate MRA-1B7, MRA-2H2 and MRA-DA2 respectively. We show that these three reagents preserve all of the properties of the original antibodies but are now essentially immortal reagents. Note that we have also expressed the MCA-1B7 Fox3/NeuN antibody in a recombinant form with a rabbit IgG backbone, RRA-1B7, extending the versatility of this useful reagent.
