EnCor Biotechnology
Mouse Monoclonal Antibody to L-Plastin Cat# MCA-5F101
Description
The MCA-5F101 antibody was made against a recombinant full length construct of the human I-Plastin sequence in NP_001138791.1 expressed in and purified from E. coli. I-plastin is also known as plastin-1 and fibrin, the later name since it was discovered as a major component of the i"fimbria" of intestinal brush border. The antibody works well on western blots of various tissues, cleanly producing the appropriate sized band. We also document the utility of this antibody for IF, ICC and IHC.
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| Name: | L-Plastin, mouse monoclonal antibody,, Cat# MCA-5F101 |
| Immunogen: | Recombinant human L-plastin expressed in and purified from E. coli |
| HGNC Name: | PLS1 |
| UniProt: | Q14651 |
| Molecular Weight: | 70kDa |
| Host: | Mouse |
| Isotype: | IgG1 heavy, κ light |
| Species Cross-Reactivity: | Human, Rat, Mouse |
| RRID: | Pending |
| Format: | Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3 |
| Applications: | WB, ICC/IF, IHC |
| Recommended Dilutions: | WB: 1:2,000. ICC/IF: 1:1,000 IHC: 1:1,000-1:2,000. |
| Storage: | Shipped on ice. Store at 4°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles. |
L-Plastin is member of the plastin family of proteins which includes two other proteins, I-plastin and T-plastin. I-plastin was originally discovered in the fimbria or brush border of the intestine localized in the actin containing microvilli (1). It was therefore originally named fimbrin and later found in many other tissues in association with actin, most notably in the stereocilia important for hearing and proprioception. The plastin proteins all have the ability to bind actin and Calcium ions, containing two EF hand sites close to the N-terminus and 4 calponin homology domains towards the C-terminus. EF hands are compact Calcium ion binding sites found in molecukes such as parvalbumin, calmodulin and many others. Pairs of calponin homology domains form binding sites for actin molecules and are found in α-actinin and many other cytoskeletal proteins.
Chromogenic Immunostaining of a 4% PFA fixed paraffin embedded rat spleen section with mouse mAb to L-plastin, MCA-5F101, dilution 1:1,000, detected with DAB (brown) using the Vector Elite ABC-HRP detection and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. This antibody performs well in staining both 4% PFA and standard NBF fixed human, mouse, and rat tissues. Mouse select image for larger view.
1. Bretscher A and Weber K. Fimbrin, a new microfilament-associated protein present in microvilli and other cell surface structures. J Cell Biol
. 1980 86:335-40. doi: 10.1083/jcb.86.1.335 (1980).
2. Delanote V, Vandekerckhove J and Gettemans J. Plastins: versatile modulators of actin organization in (patho)physiological cellular processes. Acta Pharm Sin 26:769–779 (2005).
3, Shinomiya S. Plastin Family of Actin-Bundling Proteins: Its Functions inLeukocytes, Neurons, Intestines, and Cancer. Int J Cell Biol Article ID 213492, (2012).
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