EnCor Biotechnology
Mouse Monoclonal Antibody to Pma1p Cat# MCA-40B7
Description
The MCA-40B7 antibody was originally generated after injection of a yeast nuclear preparation into a mouse for monoclonal antibody production. On screening antibodies generated from the resulting cloned hybridomas, one was found to bind to the yeast plasma membrane in immunofluorescence. This antibody was subsequently found to recognize the yeast plasma membrane ATPase (Pma1p). This protein maintains the yeast plasma membrane potential and controls celluar pH by pumping protons (H+ ions) out of the cell.
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| Name: | Pma1p, mouse monoclonal, Cat# MCA-40B7 |
| Immunogen: | Yeast nuclear prep |
| HGNC Name: | NA |
| UniProt: | W1QH21 |
| Molecular Weight: | 100kDa |
| Host: | Mouse |
| Isotype: | IgG |
| Species Cross-Reactivity: | S. cerevisiae |
| RRID: | AB_2572377 |
| Format: | Concentrated hybridoma cell culture media plus 5mM NaN3 |
| Applications: | WB, reported to work for ICC/IF |
| Recommended Dilutions: | Western blot: 1:10,000. ICC/IF: 1:1,000-1:5,000 |
| Storage: | Store at 4°C for short term, for longer term at -20°C. Avoid freeze/thaw cycles. |
Pma1p is an abundant multidomain protein of yeast which is localized in the plasma membrane. It functions as a major regulator of cytoplasmic pH, by pumping protons out of the cell. It is part of the P2 subgroup of cation-transporting ATPases. Since Pma1p is a major plasma membrane protein, antibodies which bind to it such as MCA-40B7 are useful markers of yeast plasma membranes.
This antibody was raised against a yeast protein and is not recommended for immunohistochemistry.
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2. Epstein T et al. Separation of metabolic supply and demand: aerobic glycolysis as a normal physiological response to fluctuating energetic demands in the membrane. Cancer Metab 2:7 (2014). WB. PubMed: 24982758.
3. Dighe SA, Kozminski KG. Secretory Vesicles Deliver Cdc42p to Sites of Polarized Growth in S. cerevisiae. PLoS One 9:e99494 (2014). WB; Saccharomyces cerevisiae . PubMed: 24945395.
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8. Soper JH et al. {alpha}-Synuclein-induced Aggregation of Cytoplasmic Vesicles in Saccharomyces cerevisiae. Mol Biol Cell 19:1093-103 (2008). WB; Saccharomyces cerevisiae . PubMed: 18172022
9. Martínez-Muñoz GA & Kane P Vacuolar and Plasma Membrane Proton Pumps Collaborate to Achieve Cytosolic pH Homeostasis in Yeast. J Biol Chem 283:20309-19 (2008). ICC/IF; Saccharomyces cerevisiae . PubMed: 18502746.
10. Clark SW & Rose MD Arp10p is a pointed-end-associated component of yeast dynactin. Mol Biol Cell 17:738-48 (2006). PubMed: 16291862.
11. Zhang J et al. Characterization of the transport mechanism and permeant binding profile of the uridine permease Fui1p of Saccharomyces cerevisiae. J Biol Chem 281:28210-21 (2006). WB; Saccharomyces cerevisiae . PubMed: 16854981.
12. Shibagaki N & Grossman AR The role of the STAS domain in the function and biogenesis of a sulfate transporter as probed by random mutagenesis. J Biol Chem 281:22964-73 (2006). PubMed: 16754669.
13. Okamoto M, et al. Glycosylphosphatidylinositol-anchored proteins are required for the transport of detergent-resistant microdomain-associated membrane proteins, Tat2p and Fur4p. J. Biol. Chem. 281:4013-23 (2006).
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