Recombinant Full Length Human NF-L

Human NF-L sequence was based on that was NP_006149.2 which was inserted into the eukaryotic expression vector pET30a(+) which adds an N terminal His-tag and some other sequence, underlined below. This sequence includes a thrombin cleavage site (blue), an S-tag affinity peptide (red) and an enterokinase cleavage site (green).

MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EFMSSFSYEP  60
YYSTSYKRRY VETPRVHISS VRSGYSTARS AYSSYSAPVS SSLSVRRSYS SSSGSLMPSL 120
ENLDLSQVAA ISNDLKSIRT QEKAQLQDLN DRFASFIERV HELEQQNKVL EAELLVLRQK 180
HSEPSRFRAL YEQEIRDLRL AAEDATNEKQ ALQGEREGLE ETLRNLQARY EEEVLSREDA 240
EGRLMEARKG ADEAALARAE LEKRIDSLMD EISFLKKVHE EEIAELQAQI QYAQISVEMD 300
VTKPDLSAAL KDIRAQYEKL AAKNMQNAEE WFKSRFTVLT ESAAKNTDAV RAAKDEVSES 360
RRLLKAKTLE IEACRGMNEA LEKQLQELED KQNADISAMQ DTINKLENEL RTTKSEMARY 420
LKEYQDLLNV KMALDIEIAA YRKLLEGEET RLSFTSVGSI TSGYSQSSQV FGRSAYGGLQ 480
TSSYLMSTRS FPSYYTSHVQ EEQIEVEETI EAAKAEEAKD EPPSEGEAEE EEKDKEEAEE 540
EEAAEEEEAA KEESEEAKEE EEGGEGEEGE ETKEAEEEEK KVEGAGEEQA AKKKD 595

Number of amino acids: 595

Molecular weight: 67224.80
Theoretical pI: 4.69

Amino acid composition:
Ala (A) 65 10.9%
Arg (R) 37 6.2%
Asn (N) 14 2.4%
Asp (D) 30 5.0%
Cys (C) 1 0.2%
Gln (Q) 29 4.9%
Glu (E) 102 17.1%
Gly (G) 25 4.2%
His (H) 12 2.0%
Ile (I) 20 3.4%
Leu (L) 50 8.4%
Lys (K) 41 6.9%
Met (M) 15 2.5%
Phe (F) 12 2.0%
Pro (P) 11 1.8%
Ser (S) 62 10.4%
Thr (T) 24 4.0%
Trp (W) 1 0.2%
Tyr (Y) 21 3.5%
Val (V) 23 3.9%
Total number of negatively charged residues (Asp + Glu): 132
Total number of positively charged residues (Arg + Lys): 78

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 36790
Abs 0.1% (=1 g/l) 0.547, assuming all pairs of Cys residues form cystines

Ext. coefficient 36790
Abs 0.1% (=1 g/l) 0.547, assuming all Cys residues are reduced