Recombinant Human NF-L Coil II 256-400

/in , , /by

Human NF-L sequence was based on that in NP_006149.2 which was inserted into the eukaryotic expression vector pET30a(+) which adds an N terminal His-tag and some other sequence, underlined below. This sequence includes a thrombin cleavage site (blue), an S-tag affinity peptide (red) and an enterokinase cleavage site (green). This construct expresses the entire α-helical Coil II region, which includes the epitopes for the mouse monoclonal antibodies utilized in the Uman-NF-Light™, the NF-L Simoa™ assay and others. This construct is therefore an excellent standard for these assays.


MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EFAALKDIRA  60
QYEKLAAKNM QNAEEWFKSR FTVLTESAAK NTDAVRAAKD EVSESRRLLK AKTLEIEACR 120
GMNEALEKQL QELEDKQNAD ISAMQDTINK LENELRTTKS EMARYLKEYQ DLLNVKMALD 180
IEIAAYRKLL EGEETRL 197

Number of amino acids: 197

Molecular weight: 22,395.19 Da

Theoretical pI: 5.46

Amino acid composition:
Ala (A) 25 12.7%
Arg (R) 12 6.1%
Asn (N) 8 4.1%
Asp (D) 15 7.6%
Cys (C) 1 0.5%
Gln (Q) 8 4.1%
Glu (E) 23 11.7%
Gly (G) 7 3.6%
His (H) 7 3.6%
Ile (I) 7 3.6%
Leu (L) 20 10.2%
Lys (K) 18 9.1%
Met (M) 9 4.6%
Phe (F) 4 2.0%
Pro (P) 2 1.0%
Ser (S) 11 5.6%
Thr (T) 10 5.1%
Trp (W) 1 0.5%
Tyr (Y) 4 2.0%
Val (V) 5 2.5%

Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 30

Extinction coefficients:

Ext. coefficient 11460
Abs 0.1% (=1 g/l) 0.512, assuming all pairs of Cys residues form cystines

Ext. coefficient 11460
Abs 0.1% (=1 g/l) 0.512, assuming all Cys residues are reduced