December 2023 News
We now have new data on two rabbit polyclonal antibodies to microtubule associated protein 2, RPCA-MAP2A/B and a new antibody RPCA-MAP2D. The MAP2 gene produces several different sized proteins by alternate transcription. MAP2C and MAP2D are lower molecular weight isotypes which are expressed early in development and are around 70kDa molecular size when run on SDS-PAGE. MAP2A and MAP2B isotypes are expressed later in development and on SDS-PAGE run at about 220kDa, due to the inclusion of large sequence inserts which produce the MAP2 “projection domains”. Projection domains can be seen in the electron microscope as fine protrusions from the side of microtubules in neuronal perikary and dendrites. Since MAP2C and MAP2D sequences are included in the MAP2A and MAP2B the new RPCA-MAP2D antibody will recognize all forms of MAP2, while RPCA-MAP2A/B will recognize only the mature forms of the protein. Both antibodies work well on western blots, IF, ICC and IHC.
We went to the Society for Neuroscience Meetimg in Washington DC, 11th to 15th November. Many people visited us at Booth 139 which was easy to remember as it is 30, 31, 32. We gave out the usual pens, posters, flashlights, postcards and antibody samples, and also making available more of our Santiago Ramon-Y-Cajal and Count Camillo Golgi laser cut images.
