March 2019 News

We continue with our program of epitope mapping our most widely used mouse monoclonal antibodies. We now add epitope information to the data sheet for our widely used monoclonal antibody to ubiquitin, MCA-Ubi-1. The epitope for this antibody maps to a peptide which is conserved in the ubiquitin molecule expressed in every important model organism from macaque to yeast, see here. We also mapped the epitopes for our excellent HSP60 MCA-1C7 and myelin basic protein MCA-7G7 antibodies and we provide more information about another myelin basic protein monoclonal with an epitope distinct from MCA-7G7, MCA-7D2. We also mapped the epitopes for our two mouse monoclonal antibodies to GFP, MCA-1F1 and MCA-3B11, to the N-terminus of the molecule. We also show that both antibodies recognize the two most widely used GFP proteins, AcGFP and eGFP. Finally we epitope mapped our two MAP tau mouse monoclonal antibodies, MCA-2E9. and MCA-5B10, to two distinct epitopes towards the C-terminus of human MAP tau molecules. The epitopes are expressed in all forms of tau and so both antibodies will recognize all tau isoforms on western blots and in IHC. The amino acid sequences including the epitopes are extremely conserved so the two antibodies will be usable in studies of essentially all mammals and many other vertebrates.