October 2021 News

      We release two high quality mouse monoclonal antibodies MCA-1B63 and MCA-3H25 to the S-tag, a 15 amino acid sequence originally identified as part of the N-terminal sequence of secreted bovine pancreatic ribonuclease by Frederick M. Richards in the 1950s. The S-tag sequence has been incorporated into many expression vectors and so S-tag antibodies are useful for verification of the correct expression of recombinant proteins, and the peptide can also be used as an affinity tag for protein purification. We have also have kinetic data on both antibodies, have shown they specifically bind bovine pancreatic ribonuclease and that they are useful for verifying the correct molecular size of recombinant constructs containing the S-tag sequence.
      We previously made a very popular mouse monoclonal antibody to α-synuclein MCA-2A7 and more recently another mouse monoclonal to β-synuclein MCA-6A10. Since α, β and γ-synuclein are somewhat related in protein sequence, it is important to show that MCA-2A7 is specific for α-synuclein and MCA-6A10 is specific for β-synuclein. We have run the essential western blotting experiment and can show that both antibodies are completely specific for the appropriate proteins, see data under the “additional info” tag on the web page for each antibody. Since we have made recombinant full length human β and γ-synuclein proteins available in house we are now also marketing these also, as PROT-r-SNCB and PROT-r-SNCG.