August 2017 News
We release several new antibodies to important molecules. We have made a series of antibodies in goat, which have some advantages in terms of cost and reproducibility as we are able to produce very large amounts of antibody consistently. The first is an goat polyclonal antibody to Ki-67, GPCA-Ki67, an excellent marker of dividing cells. […]
August 2016 News
We release new mouse monoclonal antibodies to MECP2, the methyl CpG binding protein 2. This is an important transcriptional regulator of neurons sequence defects in which are associated with Rett syndrome, a serious neurodevelopmental disorder. These are the IgG2b MCA-5H12 and the IgG1 MCA-4E11. We release an excellent chicken antibody to the neural stem cell marker […]
June 2016 News
We release two more Cas9/CRISPR related antibodies, a rabbit polyclonal to Streptococcus pyogenes Cas9, RPCA-Cas9-AP and a mouse monoclonal, MCA-3F9 to the same protein. These can be used to confirm the expression of the most commonly used Cas9 molecule in transfection experiments.
April 2016 News
We continue to release antibodies to Cas9, the key enzyme in the CRISPR gene editing system. These are RPCA-Cas9-SA and CPCA-Cas9-SA. These polyclonal antibodies recognize the Cas9 protein from Staphylococcus aureus, which is considerably smaller than the Streptococcus pyogenes derived Cas9. Since the two Cas9 enzymes are quite different in amino acid sequence our antibody […]
March 2016 News
The life sciences have recently been revolutionized by the exploitation of the bacterial CRISPR system. CRISPR is an acronym for “Clustered Regularly Interspaced Short Palindromic Repeats”. The short palindromes are DNA sequences generated by the bacterial host, while the DNA inserts between them are derived from infectious agents which have previously challenged the bacteria. These […]
January 2016 News
Happy New Year and all that!! We release several more novel immunoreagents. There are no less than 6 different antibodies to complement C3 made against specific subdomains of this important molecule. Three were made against a recombinant form of the netrin domain of human C3 which is at the C-terminus of the α-chain. These are […]
October 2015 News
We add two antibodies to the Heat Shock 60 (HSP60) protein, a major component of the mitochondrial membrane. These are the mouse monoclonal MCA-1C7 and a rabbit polyclonal RPCA-HSP60. Both are very clean and specific on western blots of crude extracts of cells and tissues and both stain mitochondria beautifully, as shown here. We release […]
September 2015 News
We release a novel mouse monoclonal antibody specific for microtubule associated protein 2D (MAP2D), MCA-2C4. This was raised against the purified recombinant human MAP2D, an embryonic form of this major CNS molecule. Our experiments show that this antibody also binds to MAP2C, the smallest product from the MAP2 gene. The antibody therefore will stain all […]
August 2015 News
We release three new antibodies to 2′-3′-cyclic nucleotide phosphodiesterase (CNP), a major protein of oligodendrocytes in the central nervous system. Such antibodies are therefore excellent markers of oligodendrocytes, and, in the peripheral nervous system, Schwann cells. There are two mouse monoclonals MCA-1H10 and MCA-3H5 and a rabbit polyclonal, RPCA-CNP. All work strongly and specifically on […]
Ramblings
Hi, this is the first post in an occasional series which will appear on the EnCor website. It comes out of requests by various individuals who liked to get my writings on various topics, which I was reliably informed were sometimes informative, sometimes amusing and sometimes even accurate. This all grew out of journal clubs […]
July 2015 News
We add yet more antibodies and recombinant constructs to our catalog. We release a rabbit polyclonal antibody to total MECP2, RPCA-Mecp2. MECP2 is an important transcription factor with particular activity in the nervous system. We have phosphospecific antibodies of MECP2 under development. We release an rabbit antibody to Green Fluorescent Protein, RPCA-GFP, which can be […]
May 2015 News
We release more new antibodies. To complement our calbindin antibodies we now release a new antibody to calretinin. Calbindin and calretinin are small calcium binding proteins which are each found in subsets of neurons in the CNS, and good antibodies to them are therefore useful for the identification of specific neuronal cell types. The new […]
More February 2015 News
Our immunocytochemical images continue to be used in books, journals and news articles, usually without any attribution, since we did not bother to copyright or watermark any of them. Many have been sent freely to our OEM partners and in some cases to Wikipedia. Scroll down in this very recent high profile on line article […]
New Antibodies and New Data
We release several new antibodies. These are a new mouse antibody to muscleblind like protein 1, MCA-1H1, and several to Calbindin, made in both mouse MCA-5A9, MCA-4H7 and chicken CPCA-Calb (page in preparation). We also continue to develop a better understanding of all of our current reagents. For example, we previously made antibodies to the […]
News and Brain Cell Images
We went to the Society for Neuroscience 2014 meeting in Washington DC a few weeks ago, and presented our new antibodies, ELISAs, recombinant proteins and other products, which generated some interest. However the most interest seemed to be directed at our collection of high resolution images which we presented as 24″X36″ posters. We sold some […]
Science News- STED microscopy
This years Nobel prize in Chemistry is a neat one, for the development of stimulated emission depletion (STED) microscopy. This is basically a modification of the existing confocal fluorescence microscope. Instead of scanning a specimen with a single laser to stimulate a fluorochrome the field is scanned with two lasers simultaneously. The first is the […]
New Recombinant Human Proteins and Antibodies
We have had a long time interest in the intermediate or 10nm filaments of the nervous system, antibodies to which are very useful cell type and developmental markers. There has also been considerable recent interest in the detection of these proteins as biomarkers of different kinds of CNS damage and disease. In the last few […]
Aurora A, B and C antibodies
A few months ago we made some mouse monoclonal antibodies to the interesting cell division and cancer related Aurora A, a highly conserved serine/threonine protein kinase. These seemed to work very well but we subsequently found that some of them cross-reacted with the very closely related Aurora B. We therefore went to the trouble of […]
