We publish another report concerning the breakdown products of glial fibrillary acidic protein (GFAP) which are released into blood and CSF following a variety of CNS damage and disese states, Wanner et al. BioRκiv 2025. The peer reviewed version of the report has been accepted for publication in the journal Acta Neuropathologica Communications, and will be available for free download shortly. These fragments are generated following various forms of neurodegeneration. Detection of them in blood and CSF potentially gives additional information about prognosis and progression of a variety of neurodegernative diseases in which astrocytes become compromised. The paper includes detailed epitope mapping of three EnCor mouse monoclonal antibodies to GFAP, MCA-5C10, MCA-3E10 and MCA-2A5.
We also release a new mouse monoclonal antibody to Green Fluorescent Protein (GFP). MCA-6H12. We demostrate that this antibody works well for western blotting, immunofluoresence, on formalin fixed floating sections and on paraffin embedded formalin fixed tissues from rodents transgenically expressing GFP. We have also defined the MCA-6H12 epitope and showed that the antibody binds common variants of GFP and does not cross reacrt with TdTomato or mCherry.
