Immunofluorescent analysis of a section of mouse cerebellum stained with mouse mAb to NF-L, MCA-7D1, dilution 1:5,000 in green, and costained with chicken pAb to FOX2, <a href="http://encorbio.com/products/cpca-fox2" target="_blank" rel="noopener">CPCA-FOX2</a>, dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above
Immunofluorescent analysis of a section of mouse cerebellum stained with mouse mAb to NF-L, MCA-7D1, dilution 1:5,000 in green, and costained with chicken pAb to FOX2, <a href="http://encorbio.com/products/cpca-fox2" target="_blank" rel="noopener">CPCA-FOX2</a>, dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above

EnCor Biotechnology

Mouse Monoclonal Antibody to Neurofilament NF-L (Nfl, NEFL) Cat# MCA-7D1

$250.00
Description

      MCA-7D1 antibody was made against a preparation of recombinant full length human NF-L. It binds NF-L from a variety of species including human, rat and mouse. The epitope is not fully characterized since it could not be localized using 20 amino acid nested peptides but is known to be not within the α-helical region, amino acids 88-400 of the human protein. The antibody works well for WB, IF and ICC, but is not recommended for IHC, for that we suggest MCA-DA2. We also market several other NF-L antibodies including rabbit and chicken polyclonal antibodies RPCA-NF-L and CPCA-NF-L.

Amount: 100µL of 1mg/mL
Amount: 100µL of 1mg/mL
Immunofluorescent analysis of a section of mouse cerebellum stained with mouse mAb to NF-L, MCA-7D1, dilution 1:5,000 in green, and costained with chicken pAb to the nuclear RNA binding protein FOX2, CPCA-FOX2, dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. NF-L antibody labels dendrites and axons of neuronal cells predominantly axons in white matter and basket cell axons associated with Purkinje cells. The FOX2 antibody reveals protein expressed in a subset of neurons, including the Purkinje cells.
Western blot analysis of different tissue lysates using mouse mAb to NF-L, MCA-7D1, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, and [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.

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Name: Neurofilament NF-L, mouse monoclonal, Cat# MCA-7D1
Immunogen: Full length native protein purified from pig spinal cord
HGNC Name: NEFL
UniProt: P07196
Molecular Weight: 68-70kDa
Host: Mouse
Isotype: IgG2b heavy, κ light
Species Cross-Reactivity: Human, rat, mouse, cow, pig, horse
RRID: AB_2572363
Format: Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Applications: WB, IF/ICC, IHC
Recommended Dilutions: WB: 1:2,000-1:5000. IF/ICC and IHC: 1:1000-1:5000.
Storage: Store at 4°C for short term, for longer term at -20°C

      Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other filament proteins may be included also. The major function of neurofilaments is likely to control the diameter of large axons (1). NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at 68-70kDa with some variability across species. Antibodies to NF-L like MCA-7D1 are useful for identifying neuronal cells and their processes in cell culture and sectioned material. NF-L antibody can also be useful for the visualization of neurofilament rich accumulations seen in many neurological diseases, such as Lou Gehrig’s disease (ALS), giant axon neuropathy, Charcot-Marie Tooth disease and others (2-4). Much interest has recently been focused on the detection of NF-L released from neurons into blood and CSF as a surrogate marker of primarily axonal loss in a variety of types of CNS injury and degeneration (5).



      Chromogenic immunostaining of a formalin fixed paraffin embedded rat cerebellum section with mouse mAb to NF-L, MCA-7D1, dilution 1:1,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. The MCA-7D1 antibody labels neuronal perikarya and their processes. This antibody performs well in testing with both 4% PFA and standard NBF fixed tissues. Mouse select image for larger view.

1. Hoffman et al. Neurofilament gene expression:a major determinant of axonal caliber. PNAS 84:3472-6 (1987).
2. Perrot R, et al. Review of the Multiple Aspects of Neurofilament Functions, and their Possible Contribution to Neurodegeneration. Mol. Neurobiol. 38:27-65 (2008).
3. Lépinoux-Chambaud C. Eyer J. Review on intermediate filaments of the nervous system and their pathological alterations. Histochem. Cell Biol. 140:13-22 (2013).
4. Liu Q. et al. Neurofilamentopathy in Neurodegenerative Diseases. Open Neurol. J. 5:58–62 (2011).
5. Bacioglu M, et al. Neurofilament light chain in blood and CSF as marker of disease progression in mouse models and in neurodegenerative diseases. Neuron 91:56-66 (2016).

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