Human NF-L sequence was based on that in NP_006149.2 which was inserted into the eukaryotic expression vector pET30a(+) which adds an N terminal His-tag and some other sequence, underlined below. This sequence includes a thrombin cleavage site (blue), an S-tag affinity peptide (red) and an enterokinase cleavage site (green). This construct expresses the entire α-helical […]
We release two high quality mouse monoclonal antibodies MCA-1B63 and MCA-3H25 to the S-tag, a 15 amino acid sequence originally identified as part of the N-terminal sequence of secreted bovine pancreatic ribonuclease by Frederick M. Richards in the 1950s. The S-tag sequence has been incorporated into many expression vectors and so S-tag antibodies are useful […]
You may have noticed that we recently updated our homepage, as our previous version became unstable for some unknown probably computer code related reason. We hope you like the newer version. We are glad to report that our recent paper, “TNFα increases tyrosine hydroxylase expression in human monocytes”, an early version of which was posted […]
We publish another collaboration between scientists at the University of Florida and EnCor, “TNF increases tyrosine hydroxylase expression in human monocytes” by Madison et al., BioRΧiv 10.1101/2021.04.13.439627v1, It describes in detail an ELISA developed using EnCor antibodies which can be used for quantitation of tyrosine hydroxylase (TH) levels in a variety of experimental and clinical contexts. […]
We published a preprint on the BioRΧiv server, observations on the biomarkers pNF-H and UCHL1 in the bodily fluids of patients recovering from a common type of thoracic surgery. This report “A study of the neuronal injury biomarkers pNF-H and UCHL1 in serum, CSF and urine in a cohort of thoracic endovascular aortic repair (TEVAR) […]
We release novel antibodies to one of the CasΦ, a.k.a. Cas-12j, family of enzymes. These are newly described and exceptionally compact enzymes of great potential utility in gene editing by CRISPR. Recent ecosystem and microbiome DNA sequencing and assembly characterized numerous whole genomes of “huge phages” or megaphages, see Al-Shayeb et al. Nature 578:425-431 (2020). […]
We add new data concerning our three SARS-CoV2 spike protein cell binding domain antibodies which, we now show, strongly bind to Vero cells infected with patient derived SARS-CoV2 virus. Vero cells are a cell line derived from monkey kidney which express a membrane localized ACE2 receptor very similar to the human form and are therefore […]
We add another mouse monoclonal antibody to the astrocytic and biomarker protein GFAP, MCA-3E10. This antibody was made against recombinant human GFAP and the epitope and kinetics of this antibody are known. It has utility as a component of assays designed to detect GFAP in blood, plasma and sera, which has become of potential interest […]
Like many other laboratories with expertise with antibody production we have focused some urgent attention on the SARS-Cov2 virus and the ACE2 protein which it uses to enter and infect human cells. The region of the virus spike or S-protein and the corresponding virus binding region on the ACE2 protein were recently characterized so we […]
We add more goat antibodies to high value targets, namely to 2′-5′-cyclic nucleotide phosphodiesterase (CNP), GPCA-CNP, glial fibrillary acidic protein (GFAP) GPCA-GFAP, and mCherry GPCA-mCherry. Antibodies to CNP are excellent markers of myelin sheathes, GFAP antibodies label astrocytes, neural stem cells and a few other nervous system cells while mCherry is a widely used genetically […]
