March 2023 News

      The data in our recent publication in BioRχiv preprint, entitled “Uman Type NF-L Antibodies Are Effective Reagents for the Imaging of Neurodegeneration” was edited and modified to include further data and was submitted for peer review at the journal “Brain Communications“. It has now been accepted for publication with the same title except that “NF-L” is replaced with “Neurofilament Light” in the title, we added some more data and also another coauthor. An unedited and unformatted version of the paper appeared on-line, see 10.1093/braincomms/fcad067. The same link will access the edited and formatted version of the paper which will be available soon.
      We also add new data for many of our mouse monoclonal antibodies which we have now screen for immunohistochemistry (IHC) on paraffin embedded and formalin fixed human and rodent tissues. For example our widely used mouse monoclonal antibody to c-FOS, MCA-2H2, works well on rat hippocampus highlighting a few active neurons. These cells were expressing large amounts of c-FOS and therefore activating transcription of other genes at the time the tissue was processed and with current techniques can be characterized in terms of mRNA expression and connections. This data is shown under the “Additional Info” tag on the MCA-2H2 web page. Many of our antibodies perform very well and we have posted images under the “Additional Info” tag on the relevant web pages. As expected a few antibodies did not work well and we will add “not recommended for IHC” on the web page, alonfg with details of a n alternate reagent idf we have one. So far we have data on MCA-1B7, MCA-1C51, MCA-2H2, MCA-1C7, MCA-1B11, MCA-1C7, MCA-1C51, MCA-1D2, MCA-1D4, MCA-1C7, MCA-1E3, MCA-1F1, MCA-1H10, MCA-2A7 and MCA-2A8. We are currently screening our entire panel of mouse monoclonal antibodies for utility in IHC, and will shortly post the rest of our results of this.