We add new high quality antibodies to the extreme C-terminal peptide of neurofilament NF-L, a rabbit polyclonal RPCA-NF-L-ct. This antibody was made against the extreme C-terminal peptide of rat NF-L coupled to KLH, so the epitopic region is known. This rabbit antibody binds to all mammalian NF-L molecules tested to date. After we epitope mapped our monoclonal antibodies to FOX3/NeuN we found that all of them, and also antibodies made by other companies including the original NeuN monoclonal antibody A66, bind to the same proline rich peptide at the N-terminus of the molecule (see here). We therefore made a new version of our rabbit FOX3 antibody, RPCA-FOX3, raised against this N-terminal peptide. This antibody works well for cell staining and has the advantage over other polyclonal antibodies that the exact epitopic region is known. The peptide used is 100% conserved in most mammalian and avian species so the antibody will have wide utility. Finally we release a new epitope mapped mouse monoclonal antibody to mCherry, MCA-5A6. For epitope information on this antibody see here.

We continue with our program of epitope mapping our most widely used mouse monoclonal antibodies. We now add epitope information to the data sheet for our widely used monoclonal antibody to ubiquitin, MCA-Ubi-1. The epitope for this antibody maps to a peptide which is conserved in the ubiquitin molecule expressed in every important model organism from macaque to yeast, see here. We also mapped the epitopes for our excellent HSP60 MCA-1C7 and myelin basic protein MCA-7G7 antibodies and we provide more information about another myelin basic protein monoclonal with an epitope distinct from MCA-7G7, MCA-7D2. We also mapped the epitopes for our two mouse monoclonal antibodies to GFP, MCA-1F1 and MCA-3B11, to the N-terminus of the molecule. We also show that both antibodies recognize the two most widely used GFP proteins, AcGFP and eGFP. Finally we epitope mapped our two MAP tau mouse monoclonal antibodies, MCA-2E9. and MCA-5B10, to two distinct epitopes towards the C-terminus of human MAP tau molecules. The epitopes are expressed in all forms of tau and so both antibodies will recognize all tau isoforms on western blots and in IHC. The amino acid sequences including the epitopes are extremely conserved so the two antibodies will be usable in studies of essentially all mammals and many other vertebrates.

We now have a catalog ready for downloading, select here. It’s a big catalog with a lot of high resolution images, so downloading may take some time. Also our founder was recently interviewed at the new Cade Museum for Creativity and Invention in Gainesville Florida. The interview was about the history of EnCor biotechnology and this can be heard on various on line platforms including Buzzsprout, which can be accessed here.

We release two new goat polyclonal antibodies to add to our goat antibody to MAP2, GPCA-MAP2, namely to the mesenchymal and CNS intermediate filament protein vimentin, GPCA-Vim and to green fluorescent protein GPCA-GFP. Also mouse monoclonals to GFP MCA-3B11 and MCA-1F1, also a rabbit polyclonal antibody to the same protein RPCA-GFP. Also purified recombinant GFP PROT-r-AcGFP-GFP, the form of GFP expressed in a variety of widely used expression vectors. Also new antibodies to ankyrin 3, also known as ankyrinG, a protein expressed in the axonal initial segment and the nodes of Ranvier, made in mouse, rabbit and chicken, MCA-2A8, RPCA-ANK3 and CPCA-ANK3. Also new antibodies to fatty acid binding protein 7, also known as brain lipid binding protein, made in mouse MCA-2A84, and rabbit RPCA-FABP7. Also we add two new antibodies to tyrosine hydroxylase, an enzyme heavily expressed in all catecholaminergic neurons, MCA-4H2 and RPCA-TH, to add to the chicken antibody CPCA-TH we released in June. Finally another mouse monoclonal antibody to neurofilament subunit NF-L MCA-1B11, and a novel antibody to the neuronal stem cell marker Nestin, made in chicken CPCA-Nestin.